Open AccessMercurated Dextran Column Chromatography for Fractionating Mononucleotides
Author(s) -
Dieter W. Gruenwedel,
Jianchang Fu
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.9.2002
Subject(s) - chromatography , chemistry , column chromatography , dextran , nucleotide , polysaccharide , elution , biochemistry , gene
Mercurated dextran (polysaccharide) was found to fractionate mononucleotides according to their affinity for organomercurial Hg+ . The nucleotides are released from the chromatographic column in the sequence CMP, AMP, GMP, and TMP when borate buffer of increasing pH is used as an eluent.