Open AccessIsolation of a 5S RNA-Protein Complex from Mammalian Ribosomes
Author(s) -
Günter Blobel
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.8.1881
Subject(s) - ribosome , rna , eukaryotic large ribosomal subunit , 5s ribosomal rna , ribosomal rna , ribonucleoprotein , 50s , biochemistry , protein subunit , ribosomal protein , eukaryotic ribosome , biology , protein biosynthesis , microbiology and biotechnology , chemistry , gene
5S RNA is removed from the large ribosomal subunit of both rat liver and rabbit reticulocyte ribosomes by treatment with EDTA to remove magnesium ions. The RNA is removed not as a naked molecule, but in association with a single ribosomal protein (molecular weight about 35,000) as a ribonucleoprotein, sedimenting at about 7 S. Conditions for the exclusive removal of the 5S RNA-protein complex have been established. Both ribosomal subunits undergo distinct changes in their sedimentation rate, concomitant with the loss of their biological activity, when sedimented into sucrose gradients containing high concentrations of monovalent ions and low concentrations of magnesium. Under these conditions the large subunit loses 5S RNA, the 5S RNA-associated protein, and several more proteins.