Open AccessMechanism of Initiation of Repression of In Vitro Transcription of the Lac Operon of Escherichia coli
Author(s) -
Larry Eron,
Ricardo Block
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.8.1828
Subject(s) - rna polymerase , lac repressor , transcription (linguistics) , rna polymerase ii , microbiology and biotechnology , transcription factor ii d , lac operon , operon , biology , repressor , general transcription factor , rna polymerase ii holoenzyme , polymerase , promoter , transcription factor , rna , genetics , escherichia coli , gene , gene expression , linguistics , philosophy
A cyclic AMP-binding protein (CAP protein), cyclic AMP, and RNA polymerase holoenzyme are shown to initiatelac transcription at thelac promoter.Lac repressor appears to control transcription by preventing RNA polymerase and/or CAP protein from binding to thelac promoter. Results support the idea that thelac promoter is composed of two sites that interact with CAP protein and RNA polymerase holoenzyme. The promoter can be altered by mutation so that holoenzyme alone can initiatelac transcription correctly.