Open AccessEffect of Colicin E3 upon the 30S Ribosomal Subunit of Escherichia coli
Author(s) -
B. W. Senior,
I. Barry Holland
Publication year - 1971
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.5.959
Subject(s) - 30s , colicin , ribosome , ribosomal rna , 50s , rna , eukaryotic large ribosomal subunit , rnase p , ribosomal protein , 16s ribosomal rna , escherichia coli , temperature gradient gel electrophoresis , microbiology and biotechnology , biology , 5s ribosomal rna , bacteria , chemistry , biochemistry , genetics , gene
The properties of 30S ribosomal subunits from untreated and from colicin E3-treated (E3-30S) bacteria have been compared. Polyacrylamide gel electrophoresis of ribosomal proteins revealed no difference, but several studies indicated that the 16S RNA from E3-30S particles was modified. E3-16S RNA showed slightly increased resistance to heat-induced degradation and had a reduced (15 S) sedimentation coefficient on sucrose gradients. Fingerprint analyses of E3-16S RNA revealed that the 3'-terminus of the molecule had been deleted. It was concluded that a primary effect of colicin E3 is the activation of a highly specific RNase that degrades 30S ribosomal RNA in situ, and that the resulting fragment(s) are probably retained within the 30S particle.
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