Mucopolysaccharide and Protein—Polysaccharide of a Transplantable Rat Chondrosarcoma | Zendy

Haing U. Choi | Zendy; Karl Meyer | Zendy; Richard L. Swarm | Zendy

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Mucopolysaccharide and Protein—Polysaccharide of a Transplantable Rat Chondrosarcoma

Author(s) -

Haing U. Choi,

Karl Meyer,

Richard L. Swarm

Publication year - 1971

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.68.5.877

Subject(s) - keratan sulfate , chemistry , chondroitin sulfate , chondroitin , polysaccharide , biochemistry , guanidine , proteolysis , hyaluronic acid , glycosaminoglycan , sulfate , glucuronic acid , sulfation , cartilage , chromatography , biology , organic chemistry , enzyme , anatomy

Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein-polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidine.HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein-polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage.

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