Open AccessDouble-Stranded Poliovirus RNA Inhibits Initiation of Protein Synthesis by Reticulocyte Lysates
Author(s) -
Ellie Ehrenfeld,
Tim Hunt
Publication year - 1971
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.5.1075
Subject(s) - poliovirus , reticulocyte , protein biosynthesis , rna , hela , biology , in vitro , cell free system , microbiology and biotechnology , cytoplasm , biochemistry , virus , chemistry , virology , gene
Infection of HeLa cells with poliovirus results in an inhibition of host-cell protein synthesis. Cytoplasmic extracts from infected cells contian an activity that inhibits initiation of protein synthesisin vitro by rabbit reticulocyte lysates. This inhibitory activity has been purified and characterized, and is shown to reside in poliovirus double-stranded RNA. The intact double-stranded molecule is not essential for inhibitory activity.
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