Open AccessIdentification of 1,25-Dihydroxycholecalciferol, a Form of Vitamin D 3 Metabolically Active in the Intestine
Author(s) -
Michael F. Holick,
Heinrich K. Schnoes,
Hector F. DeLuca
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.4.803
Subject(s) - metabolite , chemistry , trimethylsilyl , vitamin , cholecalciferol , active metabolite , mass spectrometry , biological activity , vitamin d and neurology , biochemistry , ether , chromatography , biology , organic chemistry , endocrinology , in vitro
A biologically active vitamin D3 metabolite (“peak V metabolite”) more polar than 25-hydroxycholecalciferol has been isolated from chicken intestines in pure form as a mono(trimethylsilyl)ether derivative. The molecule has been identified as 1,25-dihydroxycholecalciferol by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemical reactions.