Open AccessIsolation of Transducing Particles of ϕ80 Bacteriophage That Carry Different Regions of the Escherichia coli Genome
Author(s) -
Robert Press,
Nicolas Glansdorff,
Philip Miner,
Johann de Vries,
Robert J. Kadner,
Werner K. Maas
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.4.795
Subject(s) - bacteriophage , genome , biology , escherichia coli , genetics , gene , chromosome , circular bacterial chromosome , plasmid , isolation (microbiology) , strain (injury) , microbiology and biotechnology , anatomy
It has been possible to mate two strains harboring F-prime (F′) factors and to isolate from such matings rare recombinants that behave as though the two episomes had fused. Thus, two genes not previously linked may be brought into close proximity. An F′ factor carrying the attachment site for ϕ80 was fused with one carrying themet-ppc-arg region of the chromosome. Lysogenization of such a strain, followed by induction, led to the isolation of ϕ80arg + and ϕ80met + transducing phages. This technique may be utilized as a general method for joining diverse bacterial genes to the genome of phage ϕ80.