Inhibition of the Metabolism of 25-Hydroxycholecalciferol by Actinomycin D and Cycloheximide | Zendy

Yoko Tanaka | Zendy; Hector F. DeLuca | Zendy

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Inhibition of the Metabolism of 25-Hydroxycholecalciferol by Actinomycin D and Cycloheximide

Author(s) -

Yoko Tanaka,

Hector F. DeLuca

Publication year - 1971

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.68.3.605

Subject(s) - cycloheximide , metabolism , hydroxylation , dactinomycin , enzyme , biochemistry , cholecalciferol , chemistry , biology , vitamin , protein biosynthesis

Actinomycin D (or cycloheximide) administered prior to radioactive 25-hydroxycholecalciferol blocks the metabolism of 25-hydroxycholecalciferol to polar metabolites that accumulate in intestinal tissue, while it does not prevent the 25-hydroxylation of vitamin D(3) in the liver. Actinomycin D given after radioactive 25-hydroxycholecalciferol does not inhibit 25-hydroxycholecalciferol metabolism. These results indicate that 25-hydroxycholecalciferol must interact with the nuclei of cells to bring about the production of an enzyme(s) that converts it to its polar metabolites.

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