Expression of H-2 and Moloney Leukemia Virus-Determined Cell-Surface Antigens in Synchronized Cultures of a Mouse Cell Line | Zendy

Matko Cikes | Zendy; Sten Friberg | Zendy

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Expression of H-2 and Moloney Leukemia Virus-Determined Cell-Surface Antigens in Synchronized Cultures of a Mouse Cell Line

Author(s) -

Matko Cikes,

Sten Friberg

Publication year - 1971

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.68.3.566

Subject(s) - colcemid , antigen , murine leukemia virus , biology , virus , virology , cell culture , leukemia , microbiology and biotechnology , mitosis , immunofluorescence , cell cycle , cell , bone marrow , in vitro , antibody , immunology , genetics

A mouse cell line derived from bone marrow (JLS-V9) was infectedin vitro with Moloney leukemia virus. After the cell-surface antigens specified by this virus appeared, cells were synchronized in mitosis by a short treatment with colcemid. The expression of H-2- and virus-determined surface antigens was monitored during one cell cycle by an indirect membrane-immunofluorescence test. The highest proportion of antigen-positive cells was found during the G1 period; the proportion dropped as the cells entered the S period, and remained low until they entered the G1 period of the next cycle. The H-2- and virus-determined surface antigens were temporally coexpressed.

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