Open AccessKaryological Properties of Human-Mouse Somatic Hybrids
Author(s) -
Jacques Jami,
Simone Grandchamp
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.12.3097
Subject(s) - sendai virus , ploidy , biology , karyotype , hybrid , somatic cell , cell fusion , wi 38 , genetics , somatic fusion , chromosome , microbiology and biotechnology , virology , virus , cell culture , gene , botany
Karyological examinations performed 24 hr to 11 days after Sendai virus-induced fusion of mouse cells of the permanent line 3T3 and human diploid cells, suggest that hybrids with extensive loss of human chromosomes derive from fusion involving all the chromosomes of both parental cells. Examination of 39 independent clones isolated from two different crosses between L mouse cells and cells of two permanent human lines, D98/AH2 and VA2, show that the karyotypes of human-mouse hybrids are a function of the interaction between the parental cells: the mouse chromosomal complement may be doubled or not. For the first time, viable hybrids that have retained almost all of the human chromosomes have been isolated.