Open AccessDetermination of the Gene Sequence of Poliovirus with Pactamycin
Author(s) -
Donald F. Summers,
Jr Jv Maizel
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.11.2852
Subject(s) - cistron , poliovirus , coat protein , biology , virus , rna , gene , virology , peptide sequence , microbiology and biotechnology , genetics
By examination of the virus-specific polypeptides formed after the addition of pactamycin, an inhibitor of protein chain initiation, to infected cells, it has been possible to tentatively locate the virus coat proteins at the amino terminus of the large, virus-specific protein precursor, and, therefore, to assign the coat protein cistron to the 5′ end of the RNA.