Open AccessAppearance of T-cell Markers in Bone Marrow Rosette-Forming Cells after Incubation with Thymosin, a Thymic Hormone
Author(s) -
JeanFrançois Bach,
Mireille Dardenne,
Allan L. Goldstein,
Anirban Guha,
Abraham White
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.11.2734
Subject(s) - thymosin , incubation , spleen , rosette (schizont appearance) , bioassay , bone marrow , thymectomy , biology , endocrinology , cell , hormone , in vitro , medicine , rosette formation , in vivo , incubation period , immunology , microbiology and biotechnology , antibody , biochemistry , myasthenia gravis , genetics
After incubation with thymosin, a thymic hormone, normal bone marrow rosette-forming cells acquire T-cell characteristics, including increased sensitivity to azathioprine, anti-lymphocyte serum, and anti-theta serum. This activity of thymosin provides a new sensitive and reproducible bioassay for thymosin, and is well correlated with anin vivo graft-versus-host assay. In addition, incubation of spleen cells from adult thymectomized mice with thymosinin vitro restores to normal their diminished sensitivity to azathioprine and anti-lymphocte serum.