Open AccessDNA Replication and Proteins That Bind to DNA
Author(s) -
Warren E. Masker,
Helen Eberle
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.10.2549
Subject(s) - dna , nalidixic acid , dna synthesis , thymine , dna replication , escherichia coli , gel electrophoresis , biology , biochemistry , microbiology and biotechnology , chemistry , gene
Cells ofEscherichia coli 15T- were exposed to nalidixic acid or starved for thymine to inhibit DNA synthesis while protein synthesis was allowed to continue. These treatments are known to allow subsequent DNA synthesis to continue for many hours in the absence of protein synthesis. The proteins extracted from these cells that bind to DNA were examined by DNA-cellulose chromatography and disc-gel electrophoresis. One or two species were found to be absent, or to be present in greatly reduced amounts, under these conditions.