Open AccessActivation and Isolation of Hamster-Specific C-Type RNA Viruses from Tumors Induced by Cell Cultures Transformed by Chemical Carcinogens
Author(s) -
Aaron E. Freeman,
Gary J. Kelloff,
Raymond V. Gilden,
William T. Lane,
Ansel P. Swain,
Robert J. Huebner
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.10.2386
Subject(s) - hamster , biology , cell culture , carcinogen , rna , virology , virus , cell , mesocricetus , embryo , oncovirus , microbiology and biotechnology , genetics , gene
Cell cultures of Syrian hamster embryo were treated for 7 days with selected chemicals. Certain cultures were morphologically transformed by three different chemical preparations and yielded cell lines that subsequently produced malignant tumors in hamsters. Although the cell lines were negative for infectious virus before inoculation into animals, hamster-specific C-type RNA virus was isolated from tumors or from cell lines derived from the tumors. Since infectious C-type viruses are usually not demonstrable in hamster tissues of normal or tumor origin, we conclude that the chemical treatment and activation of the viruses are related events.