Open AccessProduction of 19S IgM Antibodies with Restricted Heterogeneity from Sharks
Author(s) -
Clem Lw,
Leslie Ga
Publication year - 1971
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.68.1.139
Subject(s) - antibody , immunoglobulin light chain , biology , microbiology and biotechnology , immunology
Two of three nurse sharks,Ginglymostoma cirratum , immunized with streptococcal A-variant vaccine, produced over 7 mg/ml serum of 19S IgM antibody to the group-specific carbohydrate. The light chains of this specific antibody from one shark were separated into multiple bands by disc electrophoresis. The light chains from the other antibody-producing animal were much less heterogeneous and gave only one predominant band.