Mitotic Separation of Two Human X-Linked Genes in Man—Mouse Somatic Cell Hybrids | Zendy

O. J. Miller | Zendy; Peter R. Cook | Zendy; P. Meera Khan | Zendy; S. Shin | Zendy; M. Siniscalco | Zendy

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Mitotic Separation of Two Human X-Linked Genes in Man—Mouse Somatic Cell Hybrids

Author(s) -

O. J. Miller,

Peter R. Cook,

P. Meera Khan,

S. Shin,

M. Siniscalco

Publication year - 1971

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.68.1.116

Subject(s) - hypoxanthine guanine phosphoribosyltransferase , biology , somatic cell , genetics , hypoxanthine phosphoribosyltransferase , microbiology and biotechnology , gene , phosphoribosyltransferase , somatic fusion , chromosome , mitosis , ploidy , mitotic crossover , karyotype , mutation , mutant

SIX INTERSPECIFIC SOMATIC HYBRID CELL LINES WERE DERIVED FROM A MOUSE LINE DEFICIENT IN HYPOXANTHINE: guanine phosphoribosyltransferase (HGPRT) and human diploid cells with normal enzyme activity. Human HGPRT was present in all six hybrids and the clones derived from them. However, in two of the six, and in some clones from another two, human glucose-6-phosphate dehydrogenase (G6PD) was absent. Since the structural loci for both these enzymes are X-linked in man, these findings suggest that these two loci have separated quite frequently through chromosome breakage and that they must be rather far apart on the X chromosome.

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