Open AccessStructure and Function of Bacterial Ribosomes, XI. Dependence of 50S Ribosomal Assembly on Simultaneous Assembly of 30S Subunits
Author(s) -
H Nashimoto,
Masayasu Nomura
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.3.1440
Subject(s) - 30s , 50s , ribosome , ribosomal rna , ribosomal protein , escherichia coli , protein subunit , mutant , eukaryotic large ribosomal subunit , biology , chemistry , genetics , rna , gene
Some spectinomycin-resistant mutants ofEscherichia coli are cold-sensitive. They are unable to assemble both 30S and 50S ribosomal subunits at low temperatures. They accumulate two kinds of incomplete particles, related to 30S and 50S subunits respectively. A single mutation, causing an alteration in a 30S ribosomal component, is responsible for these phenotypes. These results show that assembly of 50S subunitsin vivo is dependent on simultaneous assembly of 30S subunits. On the other hand, the assembly of 30S subunits appears to be independent of 50S assembly.