Interallelic Complementation in Hybrid Cells Derived from Human Diploid Strains Deficient in Galactose-1-Phosphate Uridyl Transferase Activity | Zendy

Henry L. Nadler | Zendy; Claramma M. Chacko | Zendy; Martin Rachmeler | Zendy

Open Access

Interallelic Complementation in Hybrid Cells Derived from Human Diploid Strains Deficient in Galactose-1-Phosphate Uridyl Transferase Activity

Author(s) -

Henry L. Nadler,

Claramma M. Chacko,

Martin Rachmeler

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.67.2.976

Subject(s) - complementation , ploidy , transferase , galactosemia , biochemistry , enzyme , microbiology and biotechnology , biology , enzyme assay , galactose , chemistry , gene , phenotype

Galactose-1-phosphate uridyl transferase activity has been demonstrated in hybrid cells formed from the fusion of human diploid fibroblasts obtained from different patients with galactosemia. The enzyme produced by the hybrid cells was similar to the normal enzyme in regard to K(m), pH optimum, and electrophoretic mobility on starch gel, but differed in specific activity, V(max) and thermal stability. This is the first reported example of interallelic complementation in fused human diploid cells.

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