Open AccessOn the Process of Cellular Division in Escherichia coli , V. Incorporation of Deoxynucleoside Triphosphates by DNA Thermosensitive Mutants of Escherichia coli Also Lacking DNA Polymerase Activity
Author(s) -
José Mordoh,
Yukinori Hirota,
François Jacob
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.2.773
Subject(s) - dnaa , escherichia coli , dna polymerase , mutant , dna polymerase ii , dna polymerase i , dna , dna clamp , microbiology and biotechnology , dnab helicase , dna replication , dnag , biology , polymerase , dna synthesis , primase , biochemistry , chemistry , circular bacterial chromosome , eukaryotic dna replication , polymerase chain reaction , gene , helicase , rna , reverse transcriptase
Double mutants ofEscherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes DNA polymerase activity. Incorporation of labeled deoxynucleoside triphosphates was studied in these mutants using toluene-treated cells. This incorporation was found to be exactly correlated with the capacity of the mutants to synthesize DNAin vivo .