DNA Synthesis by RNA-Containing Tumor Viruses | Zendy

Edward M. Scolnick | Zendy; Stuart A. Aaronson | Zendy; George J. Todaro | Zendy

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DNA Synthesis by RNA-Containing Tumor Viruses

Author(s) -

Edward M. Scolnick,

Stuart A. Aaronson,

George J. Todaro

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.67.2.1034

Subject(s) - rna , dna , enzyme , murine leukemia virus , microbiology and biotechnology , biology , polymerase , dna polymerase , biochemistry , rna polymerase , virology , chemistry , virus , gene

Murine leukemia (Rauscher and Moloney strains) and sarcoma (Kirsten strain) virions, as well as the mammary tumor virus of mice, contain an RNA-dependent DNA polymerase. Optimal incorporation of deoxyribonucleoside triphosphates occurs at a critical detergent (Triton X-100) concentration (0.010-0.014%). At higher than optimal detergent concentrations the virion is seen to be disrupted and enzyme activity is lost. The virion, enzymatic activity, and newly synthesized DNA all cosediment in a sucrose gradient. Thus far the enzymatic activity has been found only in RNA viruses that have oncogenic properties.

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