Open AccessChromosomal Distribution of Rapidly Reannealing DNA in Drosophila melanogaster
Author(s) -
Peter M. M. Rae
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.2.1018
Subject(s) - euchromatin , heterochromatin , dna , microbiology and biotechnology , biology , chromosome , drosophila melanogaster , genetics , gene , chromatin
Cytological hybridization has been used to localize fractions of rapidly reannealing DNA in salivary chromosomes ofDrosophila melanogaster . Complementary RNA of high specific activity was transcribed from hydroxyapatite-fractionated rapidly reannealing sequences and from selected buoyant-density fractions of total DNA. It was then hybridized to chromosome squashes after denaturation of DNA in NaOH. Highly „repeated” DNA sequences were detected over much of the chromosome, but were concentrated in chromocentric heterochromatin. A family of sequences with a low percentage of guanosine plus cytidine was highly concentrated in a particular region within the chromo-center. One „euchromatic” region near the tip of chromosome arm 3L also exhibited a concentration of repeated sequences.