Open AccessThe Rolling Circle for ϕX DNA Replication, III. Synthesis of Supercoiled Duplex Rings
Author(s) -
David Dressler,
John S. Wolfson
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.1.456
Subject(s) - duplex (building) , asymmetry , dna supercoil , thymidine , rolling circle replication , dna , dna synthesis , dna replication , ring (chemistry) , replication (statistics) , stereochemistry , biophysics , chemistry , crystallography , biology , physics , biochemistry , virology , organic chemistry , quantum mechanics
During ϕX duplex ring synthesis, the first supercoils to acquire radioactivity after the addition of tritiated thymidine are labeled only in their negative strands. In longer pulses, this asymmetry of labeling progressively disappears. This finding supports the rolling circle model for DNA replication due to the structural asymmetry of its replicating intermediate, but is not predicted by the Cairns or Yoshikawa models.