DNA and Gene Therapy: Uncoating of Polyoma Pseudovirus in Mouse Embryo Cells | Zendy

J V Osterman | Zendy; Anna Waddell | Zendy; H. Vasken Aposhian | Zendy

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DNA and Gene Therapy: Uncoating of Polyoma Pseudovirus in Mouse Embryo Cells

Author(s) -

J V Osterman,

Anna Waddell,

H. Vasken Aposhian

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.67.1.37

Subject(s) - embryo , dna , biology , gene , microbiology and biotechnology , virology , polyoma virus , genetic enhancement , virus , cell culture , biochemistry , genetics

Pseudovirions (host DNA fragments encapsidated by polyoma virus-coat protein) and polyoma virions are produced after infection of primary mouse embryo cells with polyoma virus. Purified pseudovirions are adsorbed to, and uncoated by, mouse embryo cells. The evidence for this uncoating is based on the conversion by pancreatic DNase of the uncoated pseudovirus DNA from an acid-insoluble to an acid-soluble form. The implications of these experiments to the eventual use of DNA for gene therapy are discussed.

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