Open AccessDNA-Dependent RNA-Directed Protein Synthesis In Vitro , IV. Peptide Analysis of an In Vitro and In Vivo ϕX174 Structural Protein
Author(s) -
David H. Gelfand,
Masaki Hayashi
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.1.13
Subject(s) - in vitro , biology , hydrolysate , bacteriophage , in vivo , biochemistry , peptide , microbiology and biotechnology , trypsin , dna , protein biosynthesis , escherichia coli , enzyme , hydrolysis , gene
An antibody-precipitable structural protein from the bacteriophage ϕX174 was purified from anin vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from the bacteriophage. The tryptic hydrolysate was subjected to high resolution peptide chromatography which revealed essentially identical patterns for the ϕX174 proteins synthesizedin vitro andin vivo .