Open AccessIMMUNOASSAY OF PLASMA VASOPRESSIN IN MAN
Author(s) -
Gary Robertson,
Lester A. Klein,
Jesse Roth,
Phillip Görden
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.4.1298
Subject(s) - vasopressin , arginine , immunoassay , sephadex , radioimmunoassay , chromatography , chemistry , endogeny , endocrinology , medicine , biology , antibody , biochemistry , enzyme , immunology , amino acid
A radioimmunoassay for arginine vasopressin has been developed that is sensitive to the hormone at 1 pg/ml. When plasma was filtered on G-25 Sephadex, immunoreactivity was detected in three regions. Endogenous arginine vasopressin was the third and smallest of the peaks and was recovered just after the salt. By combining gel filtration with immunoassay, we have made precise measurements of arginine vasopressin in a few milliliters of plasma.