Open AccessIn Vitro TRANSCRIPTION OF A TRANSFER RNA GENE
Author(s) -
Violet Daniel,
S Sarid,
Jacques S. Beckmann,
Uriel Z. Littauer
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.4.1260
Subject(s) - transfer rna , polynucleotide , transcription (linguistics) , microbiology and biotechnology , escherichia coli , dna , rna polymerase , rna , biology , gene , in vitro , polymerase , biochemistry , chemistry , linguistics , philosophy
TheEscherichia coli tRNATyr gene carried by the ϕ80psu3 + transducing phage was transcribedin vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNATyr -like polynucleotide chains were detected by competition with purifiedE. coli 32 P-tRNATyr for specific hybridization sites on ϕ80psu3 + DNA. Analysis by sucrose gradient centrifugation showed the tRNATyr -like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor ρ during the transcription did not significantly reduce the average size of thein vitro synthesized tRNATyr -like chains.