Open AccessSEQUENTIAL TRANSLATION OF TRINUCLEOTIDE CODONS FOR PEPTIDE BOND FORMATION, TRANSLOCATION, AND TERMINATION
Author(s) -
Richard K. Tompkins
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.4.1164
Subject(s) - puromycin , terminator (solar) , ribosome , chromosomal translocation , protein biosynthesis , biology , transfer rna , peptide bond , translation (biology) , gtp' , peptidyl transferase , chemistry , genetics , biochemistry , amino acid , messenger rna , rna , gene , enzyme , ionosphere , physics , astronomy
The addition of GUA, valyl-tRNA, T factor, GTP, and ethanol to reactions containing fMet-tRNA·AUG·ribosome intermediate results in the synthesis of fMet-valyl-tRNA which can be translocated if G factor is also present. The translocated peptidyl-tRNA can interact with puromycin to form fMet-Val-puromycin. Release of fMet-valine can also take place after translocation if a terminator trinucleotide and its specific release factor are present.