Open AccessImmunoglobulin Synthesis and Secretion, IV. Sites of Incorporation of Sugars as Determined by Subcellular Fractionation
Author(s) -
Jonathan W. Uhr,
Isaac Schenkein
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.3.952
Subject(s) - endoplasmic reticulum , microsome , glucosamine , cell fractionation , fractionation , leucine , biochemistry , chemistry , antibody , galactose , amino acid , secretion , golgi apparatus , biology , chromatography , enzyme , immunology
P3 K cells were uniformly labeled with [14 C]leucine and also for 1 hr with [3 H]leucine, [3 ]galactose, or [3 H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of radioactive immunoglobulin G in each fraction were determined. The results indicate that amino acids are incorporated in the rough endoplasmic reticulum, galactose primarily in the smooth endoplasmic reticulum, and glucosamine at both sites. A small portion of radioactivity was found in the microsomal supernatant regardless of the radioisotope employed.