Open AccessSpecific Separation of Cells on Affinity Columns
Author(s) -
Paolo TruffaBachi,
Leon Wofsy
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.3.685
Subject(s) - affinity chromatography , chromatography , membrane , filtration (mathematics) , chemistry , elution , cell , hapten , size exclusion chromatography , polyacrylamide , cell membrane , antibody , biochemistry , biology , enzyme , immunology , polymer chemistry , statistics , mathematics
Cell filtration is performed on affinity columns prepared by the chemical modification of large polyacrylamide beads. Affinity columns selectively bind cells that produce antihapten antibodies of corresponding specificity, while cells without the specific antihapten sites pass through freely and are recovered quantitatively in the eluate. The binding of the antihapten specific cells is inhibited by hapten. Such cell filtration affinity columns may be useful in specifically purifying cell populations with characteristic membrane-receptor proteins.