Open AccessIsolation of Temperature-Sensitive Mutants of L-Cells
Author(s) -
Larry H. Thompson,
Ralph Mankovitz,
Raymond M. Baker,
J. E. Till,
Louis Siminovitch,
G. F. Whitmore
Publication year - 1970
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.2.377
Subject(s) - mutant , isolation (microbiology) , thymidine , suspension culture , biology , microbiology and biotechnology , cell culture , chemistry , biochemistry , genetics , in vitro , gene
Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.
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