Open AccessComplementarity of RNA Produced by Enzymic Transcription of Native and Denatured B. subtilis DNA
Author(s) -
Era Cassuto,
Marshall D. Stein,
Erwin Chargaff
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.66.1.197
Subject(s) - rna , dna , microbiology and biotechnology , transcription (linguistics) , nuclease protection assay , rna dependent rna polymerase , biology , polymerase , chemistry , biochemistry , gene , philosophy , linguistics
This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase ofE. coli by transcription of the following DNA templates: (a ) undenaturedB. subtilis DNA (yielding N-RNA); (b ) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding L-RNA and H-RNA, respectively). The study of the hybridization behavior of the various RNA products showed that N-RNA, though able to form hybrids with either strand, hybridized with H-DNA to twice as great an extent as with L-DNA. The transcripts of the separated L and H fractions exhibited complete specificity with respect to complexing: L-RNA hybridized only with L-DNA, H-RNA only with H-DNA.