Immunochemical Measurement of Double-stranded RNA of Uninfected and Arbovirus-Infected Mammalian Cells | Zendy

Victor Stollar | Zendy; B. David Stollar | Zendy

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Immunochemical Measurement of Double-stranded RNA of Uninfected and Arbovirus-Infected Mammalian Cells

Author(s) -

Victor Stollar,

B. David Stollar

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.65.4.993

Subject(s) - rna , arbovirus , microbiology and biotechnology , biology , complement fixation test , sucrose gradient , virology , double stranded , virus , baby hamster kidney cell , chemistry , biochemistry , antibody , dna , genetics , serology , enzyme , gene

A quantitative complement fixation assay which specifically measures double-stranded RNA has been used to study this RNA extracted from uninfected and arbovirus-infected cells. The double-straned RNA of the uninfected BHK-21 cells sedimented in the 12S region in sucrose gradients. The double-stranded RNA of Sinbis virus-infected cells, as measured immunochemically, included a predominant peak at 12S, a smaller 18S peak, and polydisperse material extending into the 26-30S region. All classes of this RNA detected immunochemically showed a sharp thermal denaturation curve, and increased in amount progressively during infection, with the 12S peak predominant at all times.

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