Specific Nucleolar and Nucleoplasmic RNA Polymerases | Zendy

Robert G. Roeder | Zendy; William J. Rutter | Zendy

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Specific Nucleolar and Nucleoplasmic RNA Polymerases

Author(s) -

Robert G. Roeder,

William J. Rutter

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.65.3.675

Subject(s) - nucleolus , rna polymerase i , biology , transcription (linguistics) , polymerase , microbiology and biotechnology , rna , rna polymerase , rna polymerase ii , cell nucleus , dna , nucleus , small nuclear rna , biochemistry , rna dependent rna polymerase , gene expression , promoter , gene , linguistics , philosophy

The DNA-dependent RNA polymerase activity present in rat liver nuclei has been solubilized and purified from whole nuclei and from subnuclear fractions. As reported earlier (Roeder, R. G., and W. J. Rutter, Nature, 224, 234 (1969)), two major chromatographically distinct enzymatic species (I and II) are present in whole nuclei. Subfractionation of whole nuclei into nucleolar and nucleoplasmic fractions had little effect on the total recovery of activity. Purified nucleoli contain predominantly polymerase I, whereas the nucleoplasmic fraction is greatly enriched for polymerase II. A third minor peak of activity has also been resolved in the nucleoplasmic preparations. We conclude that the RNA polymerases are specifically localized within the nucleus and may, therefore, play specific roles in the regulation of genetic transcription.

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