Decay of Fluorescence Emission Anisotropy of the Ethidium Bromide-DNA Complex Evidence for an Internal Motion in DNA
Author(s) -
Ph. Wahl,
Jacques Paoletti,
Jean Bernard Le Pecq
Publication year - 1970
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.65.2.417
Subject(s) - ethidium bromide , dna , anisotropy , relaxation (psychology) , fluorescence , fluorescence anisotropy , brownian motion , biophysics , helix (gastropod) , chemistry , nuclear magnetic resonance , molecular physics , physics , optics , biology , biochemistry , quantum mechanics , ecology , neuroscience , snail
Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex. The amplitude of the oscillation is found to be equal to 35° and the relaxation time equal to 28 nanoseconds.
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