The Chain Growth Rate of T4 Lysozyme in vitro
Author(s) -
James M. Wilhelm,
Robert Haselkorn
Publication year - 1970
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.65.2.388
Subject(s) - lysozyme , in vitro , ribosomal rna , protein subunit , ribosomal protein , ribosome , bacteriophage , biochemistry , yield (engineering) , chemistry , biology , microbiology and biotechnology , rna , escherichia coli , gene , materials science , metallurgy
The average interval between attachment of a 30S ribosomal subunit to bacteriophage T4 lysozyme mRNA and the completion of synthesis of lysozyme protein has been measuredin vitro . The measured completion times yield rates of polypeptide chain propagation of 1.6 and 3.0 amino acids per second at 25°C and 31°C, respectively.
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