Open AccessA Study on the Transcription of the Polyoma Viral Genome
Author(s) -
James B. Hudson,
David Goldstein,
Roger Weil
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.65.1.226
Subject(s) - transcription (linguistics) , rna , dna , biology , microbiology and biotechnology , rna dependent rna polymerase , genome , gene , virology , genetics , philosophy , linguistics
The transcription of polyoma (Py) viral RNA was studied in primary mouse kidney cell cultures (a) during normal, asynchronous infection, (b) under conditions where Py-induced DNA synthesis was inhibited with 5-fluorodeoxyuridine, and (c) after the synchronized onset of Py-induced DNA synthesis. The results obtained suggest that the bulk of Py RNA transcribed after the (asynchronous or synchronized) onset of Py-induced DNA synthesis, designated as „late” Py RNA, corresponds to the polycistronic transcript of most or all of the genetic information contained in a strand of circular Py DNA. The rate at which late Py RNA is transcribed is related to the amount of free Py DNA present in the cultures. Py-infected cultures contain a second, minor component of Py RNA (of one or possibly more species), designated as „early” Py RNA. At least some of this RNA continues to be transcribed if Py-induced DNA synthesis is inhibited with 5-fluorodeoxyuridine and, possibly, also late during normal infection.