Topological Relationship of Prophage λ to the Bacterial Chromosome in Lysogenic Cells | Zendy

David Freifelder | Zendy; Matthew Meselson | Zendy

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Topological Relationship of Prophage λ to the Bacterial Chromosome in Lysogenic Cells

Author(s) -

David Freifelder,

Matthew Meselson

Publication year - 1970

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.65.1.200

Subject(s) - prophage , lysogenic cycle , dna , genetics , circular bacterial chromosome , biology , chromosome , covalent bond , microbiology and biotechnology , escherichia coli , chemistry , gene , dna replication , bacteriophage , organic chemistry

X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying λ prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of covalent circular DNA from the product show that the prophage DNA is linearly inserted into the sex factor, as predicted by Campbell's model for lysogenization.

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