Open AccessISOACCEPTING TRANSFER RNA'S OF L-M CELLS IN CULTURE AND AFTER TUMOR INDUCTION IN C 3 H MICE
Author(s) -
WeiPang Yang,
A. Hellman,
Donald H. Martin,
Kiki B. Hellman,
G. David Novelli
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.4.1411
Subject(s) - transfer rna , in vitro , cell culture , microbiology and biotechnology , rna , biology , biochemistry , tissue culture , genetics , gene
Co-chromatography in a reversed-phase column was performed for 16 aminoacyl-tRNA's prepared from L-M cells grown in serum-free suspension culture and from tumors induced in irradiated C3 H mice by subcutaneous injection of L-M cells. The results showed that between the two sources there were (1) marked differences in aspartyl-, histidyl-, phenylalanyl-, and tyrosyl-tRNA's; (2) significant quantitative differences in isoaccepting species of alanyl-, isoleucyl-, seryl-, and threonyl-tRNA's; and (3) similar to minimally different patterns of arginyl-, methionyl-, prolyl-, tryptophanyl-, valyl-, glycyl-, leucyl-, and lysyl-tRNA's. The differences were evident when synthetases prepared either from L-M cells or from the tumors were used. When the L-M tumors were brought back intoin vitro culture, their tRNA patterns were like those of L-M cells. Addition of serum to the culture medium caused the L-M cells to show very minute, but detectable, amounts of the isoaccepting tRNA's found in the tumors. The cellular mechanisms which may be related to the changes of tRNA patterns in the L-M cells are discussed.