Open AccessNUCLEAR MAGNETIC RESONANCE STUDIES OF THE STRUCTURE AND BINDING SITES OF ENZYMES, X. PREPARATION OF SELECTIVELY DEUTERATED ANALOGS OF STAPHYLOCOCCAL NUCLEASE
Author(s) -
Irving Putter,
Alfonso Barreto,
John L. Markley,
Oleg Jardetzky
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.4.1396
Subject(s) - deuterium , nuclease , chemistry , enzyme , molecule , protonation , stereochemistry , micrococcal nuclease , nuclear magnetic resonance spectroscopy , nmr spectra database , crystallography , spectral line , nuclear magnetic resonance , biochemistry , organic chemistry , dna , physics , ion , quantum mechanics , astronomy , nucleosome , histone
Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The1 H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.