Open AccessMECHANISM OF DNA CHAIN GROWTH, IV. DIRECTION OF SYNTHESIS OF T4 SHORT DNA CHAINS AS REVEALED BY EXONUCLEOLYTIC DEGRADATION
Author(s) -
Tuneko Okazaki,
Reiji Okazaki
Publication year - 1969
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.4.1242
Subject(s) - nuclease , dna , thymidine , exonuclease iii , exonuclease , dna synthesis , biophysics , chemistry , kinetics , biochemistry , degradation (telecommunications) , stereochemistry , biology , escherichia coli , dna polymerase , physics , computer science , gene , quantum mechanics , telecommunications
T4 nascent short chains labeled at their growing ends with H(3)-thymidine and uniformly with C(14)-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in the 3' to 5' direction or by B. subtilis nuclease in the 5' to 3' direction. The kinetics of release of H(3) and C(14) labels by both enzymes was consistent with the conclusion that the H(3) label is at the 3' end of the nascent short chains of both strands and that the short chains are products of discontinuous synthesis in the 5' to 3' direction along the two template strands.
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