Open AccessSUPPRESSION OF T4D LIGASE MUTATIONS BY rIIA AND rIIB MUTATIONS
Author(s) -
Hillard Berger,
Andrzej W. Kozinski
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.3.897
Subject(s) - dna ligase , mutant , microbiology and biotechnology , biology , ligase chain reaction , mutation , bacteriophage , ubiquitin ligase , dna , genetics , gene , escherichia coli , polymerase chain reaction , ubiquitin , multiplex polymerase chain reaction
The introduction ofr II mutations into polynucleotide ligaseamber mutants (gene 30) of T4D bacteriophage results in the restoration of phage growth in the nonpermissive hostE. coli B. When cells are mixedly infected withr II+ -ligase- andr II-ligase- phage, growth is reduced, indicating that ther II mutations are recessive to ther II+ alleles. Infection with ther II-ligase- double mutants results in nearly complete restoration of phage DNA synthesis and prevents the extensive degradation of parental phage DNA observed after infection with ligase- single mutants. Similar results are observed when cells are infected with ligase- mutants and chloramphenicol is added five minutes post infection.