INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC VIRUS | Zendy

Itaru Takebe | Zendy; Yoshiaki Otsuki | Zendy

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INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC VIRUS

Author(s) -

Itaru Takebe,

Yoshiaki Otsuki

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.64.3.843

Subject(s) - protoplast , tobacco mosaic virus , nicotiana tabacum , virus , inoculation , biology , virology , incubation , tobacco etch virus , plant virus , microbiology and biotechnology , botany , horticulture , biochemistry , potyvirus , gene

It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.

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