MOLECULAR HYBRIDIZATION OF RADIOACTIVE DNA TO THE DNA OF CYTOLOGICAL PREPARATIONS | Zendy

Mary Lou Pardue | Zendy; Joseph G. Gall | Zendy

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MOLECULAR HYBRIDIZATION OF RADIOACTIVE DNA TO THE DNA OF CYTOLOGICAL PREPARATIONS

Author(s) -

Mary Lou Pardue,

Joseph G. Gall

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.64.2.600

Subject(s) - dna , nuclear dna , microbiology and biotechnology , biology , dna–dna hybridization , xenopus , in situ hybridization , chemistry , biochemistry , gene , mitochondrial dna , gene expression

A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.

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