Open AccessTHE MECHANISM OF MESSENGER RNA TRANSLOCATION THROUGH RIBOSOMES
Author(s) -
William J. Culp,
Wallace L. McKeehan,
Boyd Hardesty
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.1.388
Subject(s) - ribosome , transfer rna , five prime cap , guanosine , ribosomal rna , rna , guanosine triphosphate , biochemistry , 50s , messenger rna , chemistry , enzyme , biology , microbiology and biotechnology , rna editing , gtp' , gene
The two recognized enzymatic steps involved in the extension of peptides on ribosomes of the 80S type have been studied in a highly purified transfer system derived from rabbit reticulocytes. Data presented are interpreted to reflect three ribosomal binding sites through which transfer RNA is moved in two independent enzymatic reactions each of which requires guanosine 5′-triphosphate hydrolysis. The binding enzyme facilitates translocation between the entry and acceptor ribosomal sites. Transferase II is involved in translocation between the acceptor and donor ribosomal sites. A model is proposed to account for movement of transfer RNA and messenger RNA between the ribosomal sites.