Open AccessIn vitro DIFFERENTIATION OF A MOUSE NEUROBLASTOMA
Author(s) -
David Schubert,
Susie Humphreys,
Carlo Baroni,
Melvin Cohn
Publication year - 1969
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.1.316
Subject(s) - neurofilament , microbiology and biotechnology , tyrosine hydroxylase , cytoplasm , in vitro , cell culture , tissue culture , cell , cellular differentiation , biology , neuron , electron microscope , chemistry , biochemistry , immunohistochemistry , immunology , neuroscience , enzyme , genetics , physics , gene , optics
Mouse tumor C1300 has been established in tissue culture. The cells have a round cell morphology in both the subcutaneous tumor and in suspension culture. However, when given a surface on which to attach, they send out processes up to 3 mm in length and assume the morphology of mature neurons. The attached cells are stained by the Bodian silver procedure for neurons, whereas the cells grown in suspension are not. Electron microscopy reveals that the attached cells contain neurofilaments, neurotubules, and densecore vesicles indicative of nerve fibers. Both free-floating and attached cells have tyrosine hydroxylase activity characteristic of sympathetic nervous tissue. Apparently cell attachment can induce morphological differentiation from an anaplastic round cell to a cell which has many properties of a mature neuron.
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