Open AccessTHE PROCESS OF INFECTION WITH BACTERIOPHAGE ϕX174, XXX. REPLICATION OF DOUBLE-STRANDED ϕX DNA
Author(s) -
Rolf Knippers,
J. M. Whalley,
Robert L. Sinsheimer
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.1.275
Subject(s) - dna , dna replication , biology , bacteriophage , semiconservative replication , dna clamp , double stranded , biophysics , dna synthesis , nucleotide , microbiology and biotechnology , chemistry , control of chromosome duplication , genetics , polymerase chain reaction , gene , reverse transcriptase , escherichia coli
Intermediates involved in the replication of double-stranded ϕX174 RF DNA have been identified and partially characterized. Analysis of pulselabeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with “tails” and “double rings,” which could be the intermediate structures. A model is postulated for the replication process.