Open AccessTHE MECHANISM OF VIRAL CARCINOGENESIS BY DNA MAMMALIAN VIRUSES, VI. A NEW CLASS OF VIRUS-SPECIFIC RNA MOLECULES IN CELLS TRANSFORMED BY GROUP C HUMAN ADENOVIRUSES
Author(s) -
Kei Fujinaga,
Magdalena Piña,
Maurice Green
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.1.255
Subject(s) - biology , dna , rna , virology , adenovirus genome , adenoviridae , microbiology and biotechnology , virus , nucleic acid thermodynamics , adenovirus infection , gene , genome , mastadenovirus , genetics , genetic enhancement
A new class of virus-specific RNA molecules was found in cells transformed by group C human adenovirus types 2, 5, and 6. RNA isolated from virus-free rat embryo cells transformed by adenovirus 2, 5, and 6 hybridized with all group C adenovirus DNA's (adenovirus 1, 2, 5, and 6) equally well, but not appreciably with group A and B adenovirus DNA's. Most likely no viral genes common to group A, B, and C adenoviruses are transcribed in adenovirus-transformed cells. Group C adenoviruses are closely related since they share 83 to 93 per cent of their base sequences as shown by DNA-DNA homology measurements. Group C DNA's share only 10 to 26 per cent of their base sequences with group A and B DNA's. Moreover, the shared sequences are not transcribed detectably in adenovirus transformed cells. Virus-specific RNA isolated from group C transformed cells contains 49 to 51 per cent G + C, but viral DNA's possess a 7 to 9 per cent higher G + C content. These differences suggest that only a portion of the viral genome with an average G + C content of 49 to 51 per cent is transcribed in group C adenovirus transformed cells.