DIABETES MELLITUS AND AGING: DIMINISHED PLATING EFFICIENCY OF CULTURED HUMAN FIBROBLASTS | Zendy

Samuel Goldstein | Zendy; John W. Littlefield | Zendy; J. Stuart Soeldner | Zendy

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DIABETES MELLITUS AND AGING: DIMINISHED PLATING EFFICIENCY OF CULTURED HUMAN FIBROBLASTS

Author(s) -

Samuel Goldstein,

John W. Littlefield,

J. Stuart Soeldner

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.64.1.155

Subject(s) - plating efficiency , diabetes mellitus , pathogenesis , in vitro , plating (geology) , biology , endocrinology , gene , inheritance (genetic algorithm) , medicine , cell culture , genetics , microbiology and biotechnology , paleontology

This work concerns the effect of age and the diabetic gene(s) on the growth capacity of skin fibroblasts in culture. Cells from normal subjects and the progeny of conjugal diabetics have similar lifespans after multiple passages in mass culture. The combined lifespansin vitro are inversely proportional to the age of the donor. When individual cells are plated, more of those from normal subjects are able to form colonies. The difference in plating efficiency is apparent when first tested after 20 generations of growth, persists at 30 and 40 generations, but disappears after 50 generations. This cell culture system should be useful in studying the inheritance of the diabetic gene(s), the pathogenesis of the diabetic state, and the relationship between aging and diabetes, both of which decrease plating efficiency.

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