In vitro SYNTHESIS OF DIFFERENT CATEGORIES OF SPECIFIC PROTEIN BY MEMBRANE-BOUND AND FREE RIBOSOMES | Zendy

M. Clelia Ganoza | Zendy; Curtis A. Williams | Zendy

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In vitro SYNTHESIS OF DIFFERENT CATEGORIES OF SPECIFIC PROTEIN BY MEMBRANE-BOUND AND FREE RIBOSOMES

Author(s) -

M. Clelia Ganoza,

Curtis A. Williams

Publication year - 1969

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.63.4.1370

Subject(s) - ribosome , endoplasmic reticulum , cytoplasm , organelle , protein biosynthesis , cell free system , membrane protein , biochemistry , in vitro , biology , vesicle , membrane , chemistry , microbiology and biotechnology , rna , gene

The free and membrane-bound ribosomes of hepatic cells were isolated and used to program protein synthesis in cell-free extracts. Immuno-electrophoretic and radioautographic analysis of the products showed that the membrane-bound ribosomes synthesized serum proteins, whereas ribosomes free of endoplasmic reticulum synthesized specific nonserum liver proteins. After synthesis, the serum proteins remained associated with membrane vesicles, whereas the other liver proteins were found free of cytoplasmic organelles.

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